Ozonated Glycerin: How to Nebulize It, Drink It, and What to Look For
By Matthew Coffman, HTMA practitioner and host of the Integrative Thoughts Podcast
Disclosure: I'm a SimplyO3 affiliate. If you buy through my links or use code ITP10, you get 10% off and I earn a small commission.
Ozonated glycerin is the ozone product I get asked about most right now, and almost every question is the same two: can you nebulize it, and can you drink it? Yes to both. Here's what it is, how people do each one, how much to start with, and what to look for before you buy a bottle.
Quick answers
What it is: vegetable glycerin that has had ozone run through it until it holds ozone-derived compounds.
Why it's different from ozone oil: glycerin mixes with water. That's what lets you nebulize it and drink it. Oil can't do either.
Nebulizing: always diluted in distilled water. Start at 2.5% (1% if you're sensitive), about 5 mL per session, once or twice a day, in a plug-in compressor nebulizer with a mask.
Drinking: mixed into water. Start at ½ teaspoon a day and work up slowly to no more than 1 tablespoon a day.
The one rule: low and slow. Start weak and only step up if you feel good.
What is ozonated glycerin?
Glycerin (glycerol) is a clear, thick, sweet liquid made from plants. To make ozonated glycerin, a manufacturer runs ozone gas through pure glycerin for a long time, often days. The ozone reacts with the glycerin and forms new compounds the glycerin holds onto. You'll see it called ozonated glycerin, ozonized glycerin, or OG. Same thing.
Ozonated glycerin vs. ozone oil: why it matters for nebulizing and drinking
Ozonated glycerin | Ozonated oil | |
Base | Vegetable glycerin | Usually olive oil |
Mixes with water | Yes | No |
Can be nebulized | Yes, diluted in distilled water | No |
Can be mixed into a drink | Yes, tastes sweet | No (capsules instead) |
On the skin | Light, absorbs fast | Thick, stays put |
Storage | Fridge | Longer shelf life |
If you want ozone in a form you can breathe as a mist or put in a glass of water, glycerin is the one. SimplyO3 has a deeper side-by-side on ozonized glycerin vs. ozone oil.
How to nebulize ozonated glycerin
Nebulizing turns diluted ozonated glycerin into a fine mist you breathe in through a mask. The amounts most people use come from ozone practitioners, including Dr. Robert Rowen's nebulizing guidelines.
What you need
A plug-in compressor nebulizer with a face mask. Not a mesh or ultrasonic one: glycerin clogs the mesh, and ultrasonic units don't mist it reliably. Plug-in units make a denser mist than battery handhelds. Most run $50 to $100. Compressor nebulizers with masks on Amazon.
Distilled water (saline also works). Never tap water.
A 1 mL syringe for the glycerin and a 10 mL syringe for the water, no needles.
A small glass for mixing.
How strong to make it
Strength | For a 5 mL cup | When |
1% | 0.05 mL glycerin + 4.95 mL water | Extra gentle, if you're sensitive |
2.5% | 0.125 mL glycerin + 4.875 mL water | Start here |
5% | 0.25 mL glycerin + 4.75 mL water | Step up after a few comfortable sessions |
10% | 0.5 mL glycerin + 4.5 mL water | Upper limit |
A session
Measure the glycerin and the water into the glass.
Swirl for 2 to 3 minutes. Glycerin doesn't want to mix with water, so keep going until it looks even.
Pour it into the nebulizer cup, attach the mask and turn it on.
Sit upright and breathe normally. Don't force deep breaths.
Run it until the cup is empty, about 10 to 15 minutes.
Rinse the cup and mask with warm water and let them dry.
Once or twice a day. Never nebulize it undiluted. If a session feels heavy, stop and go back to a weaker mix next time. Leftover mix keeps in the fridge for up to 24 hours.
This is the same thing I tell people about ozone in general: go low and slow. A lot of people are more toxic or more sensitive than they think, and starting at 1% or 2.5% and staying there for a while is smart. There's no prize for jumping to 10%.
For the full mixing table (including 10 mL batches) and step-by-step instructions, see SimplyO3's guide on how to nebulize ozonated glycerin.
How to drink ozonated glycerin
Mix it into water, or into cooled tea or coffee. It tastes sweet. Work up slowly:
Week 1: ½ teaspoon a day
Week 2: 1 teaspoon a day
After that: up to 1 tablespoon a day
Too much at once can loosen your stools. If that happens, take less. Same idea as nebulizing: start small, give your body time, and only go up if you feel good.
SimplyO3 covers drinking and every other use in how to use ozonated glycerin.
Other ways people use it
Nasal spray: mixed with saline in a nasal pump bottle, about 10% (5% if you're sensitive). Never tap water in your nose.
Mouthwash: about 10% in water. Swish for 1 to 2 minutes and spit.
Skin: about 10% mixed into your lotion or moisturizer. Patch test first.
What does the research say?
Honestly, it's early. Most of what's out there on nebulizing and drinking comes from practitioners, not big studies. The published research so far is mostly on skin and on the chemistry:
Age spots (2022). In a controlled study, 48 women applied ozonated glycerin cosmetic formulas (80 and 800 ppm) twice a day for 8 weeks. Researchers reported a decrease in melanin in their age spots compared with controls, and no adverse events. It was run by the company that makes those formulas. (Hanada et al., Journal of Cosmetic Dermatology, 2022)
Skin research (2026). A study in Cosmetics looked at ozonized glycerin and skin repair. (Ivarsson et al., 2026)
Chemistry (2026). A paper in Ozone: Science & Engineering measured the peroxide and total oxidant values of ozonated glycerin, which gives the industry a way to compare products. (Sabbah & Martínez-Sánchez, 2026)
What to look for when you buy
If you're going to breathe it or drink it, quality matters even more. You can't tell good ozonated glycerin from bad by looking at it. Here's what I'd check:
Freshness. It loses strength over time. Look for small batches or made to order, shipped fast.
A real shelf life and storage directions. No shelf life listed is a red flag.
Glass packaging. Ozone-derived compounds can react with cheap plastics.
A clean base. Pure, high-grade vegetable glycerin.
Known strength. The mixing tables above assume you know how strong your bottle is. Ask what's in it. Most brands can't tell you.
A guarantee. If a company stands behind it, they'll take it back.
The one I'd buy: Glyzine
The ozonated glycerin I point people to is Glyzine from SimplyO3. I've used SimplyO3's equipment for years, and Glyzine checks the boxes above:
About 10,000 ppm, made from USP-grade organic vegetable glycerin
Made to order in a cleanroom built to ISO Class 5 specifications, and ships within 2 business days of production
Comes in glass, with a 90-day shelf life at room temperature. Refrigerate it (36 to 46°F) to extend that, and don't freeze it.
The first ozonated glycerin with an independent LC-MS chemical profile, so there's real data on what's in the bottle
SimplyO3 publishes clear mixing guides for nebulizing and drinking that match its strength
A 30-day money-back guarantee, even if it's opened
The 2 oz bottle ($49.99) is plenty to start, since you only use a fraction of a milliliter per nebulizer session. Use code ITP10 for 10% off.
Where this fits with mineral balancing
Most of my work with HTMA is inside-out: minerals, diet, and bringing toxic load down. Ozone products can be a nice part of a routine, but they're not a replacement for the foundational work. And the more toxic someone is, the lower and slower they should start. If you want to know where your minerals stand first, that's what I do. Get an HTMA test.
This is general product-use information, not medical advice. Follow your nebulizer manufacturer's instructions, stop if you feel any discomfort, and talk with a qualified practitioner if you have a health condition.
FAQ
Can you nebulize ozonated glycerin?
Yes, but only diluted in distilled water or saline, never undiluted. Start at 2.5% (1% if you're sensitive) in a plug-in compressor nebulizer with a mask.
How much ozonated glycerin do you put in a nebulizer?
About 5 mL of mix per session. At 2.5%, that's 0.125 mL of ozonated glycerin plus 4.875 mL of distilled water. Once or twice a day.
Can you drink ozonated glycerin?
Yes. Mix it into water, or cooled tea or coffee. Start at ½ teaspoon a day and work up to no more than 1 tablespoon a day.
What kind of nebulizer works with glycerin?
A plug-in compressor (jet) nebulizer with a mask. Glycerin clogs mesh nebulizers, and ultrasonic ones don't mist it reliably.
Is ozonated glycerin the same as ozone oil?
No. Glycerin mixes with water, so you can nebulize it and drink it. Oil can't be used either way.
How should I store it?
In the fridge, in its glass bottle, not frozen. Mixed batches: fridge, and use within 24 hours.
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